Aislamiento e identificación de microorganismos ambientales del museo Tumbas Reales de Sipán

Isolation and identification of the environmental microorganism of the Tumbas Reales de Sipán museum

Contenido principal del artículo

Rocío Farro-Barbaran
Willy Mauricio Ramos-Iturregui
Sebastian Iglesias-Osores
Carmen Carreño-Farfán

Resumen

Las bacterias y los hongos son un riesgo potencial para el material arqueológico y trabajadores de los museos en Lambayeque, Perú. Con el objetivo de cuantificar la microbiología ambiental aislada del museo Tumbas Reales de Sipán, se muestrearon cuatro almacenes, con Agar Nutritivo y Agar Papa Dextrosa, incubándose a 30 °C, en aerobiosis, hasta por 7 días, para bacterias y, hasta por 10 días, para hongos. Se cuantificaron bacterias con un máximo de 2,7x104 UFC cm-2, en las superficies de pisos y paredes; 1,4x103 UFC m-3, en el aire y hongos filamentosos, con un máximo de 7,5x104 UFC cm-2, en las superficies y 9,1x104 UFC m-3, en el aire. Se aislaron e identificaron los géneros de bacterias: Bacillus, Micrococcus, Acinetobacter, Nocardia, Streptococcus, Staphylococcus y Streptomyces y los géneros de hongos filamentosos: Aspergillus, Cladosporium, Circinella, Syncephalastrum, Penicillium, Alternaria, Staphylotrichum, Cercospora, Rhizopus, Cunninghamella, Chrysosporium, Nigrospora, Stachybotrys, Gliocladium y Verticillium.

Palabras clave:

Descargas

Los datos de descargas todavía no están disponibles.

Detalles del artículo

Referencias (VER)

BARNETT, H.L.; HUNTER, B.B. 1998. Illustrated Genera of Imperfect Fungi. 4th Edition, APS Press, St. Paul. 218p.

CAVKA, M.; GLASNOVIĆ, A.; JANKOVIĆ, I.; SIKANJIĆ, P.R.; PERIĆ, B.; BRKLJACIĆ, B.; MLINARIĆ-MISSONI, E.; SKRLIN, J. 2010. Microbiological analysis of a mummy from the archeological museum in Zagreb. Collegium Antropologicum. 34(3):803-805.

CHMIEL, M.; KRAL, I.; LENART-BORON, A. 2019. Concentration and size distribution of microbial aerosol in the historical objects in Kraków as a potential health risk and biodeterioration factor. Aerobiologia. 35(4):743-758.

https://doi.org/10.1007/s10453-019-09614-x

CIFERRI, O. 2002. The role of microorganisms in the degradation of cultural heritage. Studies in Conservation. 47(Supplement-1):35-45.

https://doi.org/10.1179/sic.2002.47.supplement-1.35

COX, C.S.; WATHES, C.M. 1995. Bioaerosols handbook. crc press.

DAÂSSI, D.; ZOUARI-MECHICHI, H.; BELBAHRI, L.; BARRIUSO, J.; MARTÍNEZ, M.J.; NASRI, M.; MECHICHI, T. 2016. Phylogenetic and metabolic diversity of Tunisian forest wood-degrading fungi: a wealth of novelties and opportunities for biotechnology. 3 Biotech. 6(1):46.

https://doi.org/10.1007/s13205-015-0356-8

EYZAGUIRRE, M.P.L. 2002. Análisis científico de fibras arqueológicas. Conserva. 6(1):47-59.

FAROOQ, M.; HASSAN, M.; GULL, F. 2015. Mycobial Deterioration of Stone Monuments of Dharmarajika, Taxila. J. Microbiology & Experimentation. 2(1):29-33.

https://doi.org/10.15406/jmen.2015.02.00036

GRBIĆ, M.L.; STUPAR, M.; VUKOJEVIĆ, J.; MARIČIĆ, I.; BUNGUR, N. 2013. Molds in museum environments: Biodeterioration of art photographs and wooden sculptures. Archives of Biological Sciences. 65(3):955-962.

https://doi.org/10.2298/ABS1303955G

GU, J.D. 2003. Microbiological deterioration and degradation of synthetic polymeric materials: Recent research advances. Internal Biodeterioration and Biodegradation. 52(2):69-91.

https://doi.org/10.1016/S0964-8305(02)00177-4

GU, J.D.; FORD, T.E.; BERKE, N.S.; MITCHELL, R. 1998. Biodeterioration of concrete by the fungus Fusarium. Internal Biodeterioration and Biodegradation. 41(2):101-109.

https://doi.org/10.1016/S0964-8305(98)00034-1

GUIAMET, P.; BORREGO, S.; LAVIN, P.; PERDOMO, I.; GÓMEZ DE SARAVIA, S. 2011. Biofouling and biodeterioration in materials stored at the Historical Archive of the Museum of La Plata, Argentine and at the National Archive of the Republic of Cuba. Colloids and Surfaces B: Biointerfaces. 85(2):229-234.

https://doi.org/10.1016/j.colsurfb.2011.02.031

HÖRZ, G.; KALLFASS, M. 2000. The treasure of gold and silver artifacts from the Royal Tombs of Sipán, Peru - A study on the Moche metalworking techniques. Materials Characterization. 45(4–5):391-419.

https://doi.org/10.1016/S1044-5803(00)00093-0

KAKAKHEL, M.A.; WU, F.; GU, J.D.; FENG, H.; SHAH, K.; WANG, W. 2019. Controlling biodeterioration of cultural heritage objects with biocides: A review. Internal Biodeterioration and Biodegradation. 143(2019):104721.

https://doi.org/10.1016/j.ibiod.2019.104721

KARBOWSKA-BERENT, J.; GÓRNY, R.L.; STRZELCZYK, A.B.; WLAZŁO, A. 2011. Airborne and dust borne microorganisms in selected Polish libraries and archives. Building and Environment. 46(10):1872-1879. https://doi.org/10.1016/j.buildenv.2011.03.007

KONSA, K.; TIRRUL, I.; HERMANN, A. 2014. Wooden objects in museums: Managing biodeterioration situation. Internal Biodeterioration and Biodegradation. 86(2014):165-170.

https://doi.org/10.1016/j.ibiod.2013.06.023

LABARCA, M.; SANABRIA, N.; ARCIA, A. 2006. Patogenicidad de Pestalotiopsis palmarum Cooke, sobre plantas de vivero de palma aceitera (Elaeis guineensis Jacq.). Rev. Fac. Agron. (LUZ). 23:417-424

LIU, X.; MENG, H.; WANG, Y.; KATAYAMA, Y.; GU, J.D. 2018. Water is a critical factor in evaluating and assessing microbial colonization and destruction of Angkor sandstone monuments. Internal Biodeterioration and Biodegradation. 133(2018):9-16.

https://doi.org/10.1016/j.ibiod.2018.05.011

LÓPEZ-ARANDA, B.G.; IGLESIAS-OSORES, S.; TULLUME GONZALES, L.; CARREÑO-FARFAN, C. 2020. Caracterización microbiológica de los almacenes del museo tumbas reales de Sipán en Lambayeque, Perú. La zaranda de ideas. 18(2):136-145.

MENG, H.; KATAYAMA, Y.; GU, J.D. 2017. More wide occurrence and dominance of ammonia-oxidizing archaea than bacteria at three Angkor sandstone temples of Bayon, Phnom Krom and Wat Athvea in Cambodia. Internal Biodeterioration and Biodegradation. 117:78-88.

https://doi.org/10.1016/j.ibiod.2016.11.012

MENG, H.; LUO, L.; CHAN, H.W.; KATAYAMA, Y.; GU, J.D. 2016. Higher diversity and abundance of ammonia-oxidizing archaea than bacteria detected at the Bayon Temple of Angkor Thom in Cambodia. Internal Biodeterioration and Biodegradation. 115(2016):234-243.

https://doi.org/10.1016/j.ibiod.2016.08.021

MONTANARI, M.; MELLONI, V.; PINZARI, F.; INNOCENTI, G. 2012. Fungal biodeterioration of historical library materials stored in Compactus movable shelves. Internal Biodeterioration and Biodegradation. 75:83-88.

https://doi.org/10.1016/j.ibiod.2012.03.011

OBRUCA, S.; MAROVA, I.; MATOUSKOVA, P.; HARONIKOVA, A.; LICHNOVA, A. 2012. Production of lignocellulose-degrading enzymes employing Fusarium solani F-552. Folia Microbiologica. 57(3):221-227.

PIÑAR, G.; PIOMBINO-MASCALI, D.; MAIXNER, F.; ZINK, A.; STERFLINGER, K. 2013. Microbial survey of the mummies from the Capuchin Catacombs of Palermo, Italy: biodeterioration risk and contamination of the indoor air. FEMS Microbiology Ecology. 86(2):341–356.

https://doi.org/10.1111/1574-6941.12165

ROJAS, T.I.; AIRA, M.J.; BATISTA, A.; CRUZ, I.L.; GONZÁLEZ, S. 2012. Fungal biodeterioration in historic buildings of Havana (Cuba). Grana. 51(1):44-51.

https://doi.org/10.1080/00173134.2011.643920

SEQUEIRA, S.; CABRITA, E.J.; MACEDO, M.F. 2012. Antifungals on paper conservation: An overview. Internal Biodeterioration and Biodegradation. 74(2012):67-86.

https://doi.org/10.1016/j.ibiod.2012.07.011

SHIMADA, I.; HÄUSLER, W.; JAKOB, M.; MONTENEGRO, J.; RIEDERER, J.; WAGNER, U. 2003. Early pottery making in Northern Coastal Peru. Part IV: Mössbauer study of ceramics from Huaca Sialupe. Hyperfine Interactions. 150(1-4):125-139.

https://doi.org/10.1023/B:HYPE.0000007354.42840.ef

STERFLINGER, K.; PIÑAR, G. 2013. Microbial deterioration of cultural heritage and works of art - Tilting at windmills? Applied Microbiology and Biotechnology. 97(22):9637-9646.

https://doi.org/10.1007/s00253-013-5283-1

WARSCHEID, T.; BRAAMS, J. 2000. Biodeterioration of stone: A review. Internal Biodeterioration and Biodegradation. 46(4):343-368.

https://doi.org/10.1016/S0964-8305(00)00109-8

ZHU, H.; PHELAN, P.E.; DUAN, T.; RAUPP, G.B.; FERNANDO, H.J.S.; CHE, F. 2003. Experimental study of indoor and outdoor airborne bacterial concentrations in Tempe, Arizona, USA. Aerobiologia. 19(3-4):201-211.

https://doi.org/10.1023/B:AERO.0000006571.23160.8a

Citado por