Pilot work on rabbits inducing ischemia/reperfusion to test two therapeutic protocols Trabajo piloto en conejos induciendo el síndrome isquemia/reperfusión para el ensayo de dos protocolos terapéuticos

Abstract

In order to establish comparative parameters of the effect of two alternative therapies for the treatment of the ischemia and reperfusion syndrome in veterinary medicine, 30 New Zealand rabbits were subjected to ischemia by clamping of two segments of the small intestine, one affecting the venous irrigation and the other one with arterial-venous compromise. After one hour of ischemia two treatments were tested, ozone and glucose + insulin + potassium (GIP), the control group was not treated. The reestablishment of the circulatory flow was permitted two hours after the ischemia. Samples of the intestinal segments were taken before and one and two hours after the ischemia and one hour after the reperfusion period. To evaluate injuries at 12h post ? reperfusion, euthanasia of all animals was practiced and samples of the manipulated intestine taken. A descriptive statistic analysis and a test based on aleatory (Wald . Wolfowitz) was used, since the variable response is not continuous. Epithelial damage and transmural inflammation of the intestine sampled was found, with higher intensity of those samples with longer ischemia time and higher in those with no treatment, showing significant differences (p≤0.05).