Vitrificación de embriones bovinos producidos in vitro
Vitrification of bovine embryos produced in vitro
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En Colombia y en el mundo la producción de embriones in vitro, a nivel comercial, se ha incrementado en los últimos años. La transferencia de los mismos, se realiza, principalmente, en fresco, debido a las posibles lesiones causadas por los procesos de criopreservación. La vitrificación es una herramienta útil en tecnologías de reproducción asistida (TRA), que se emplea en medicina humana y en veterinaria, que puede ser usada como alternativa para la criopreservación de germoplasma (oocitos, espermatozoides y embriones). Pese a los grandes avances que se han realizado en el desarrollo de dicha técnica, su aplicación es escasa en la industria de producción de embriones bovinos y se emplea, básicamente, en proyectos de investigación. En esta revisión, se discuten diferentes aspectos involucrados en el proceso de vitrificación de embriones in vitro, algunos de los resultados obtenidos, las ventajas y las principales limitantes de la misma.
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ARAV, A. 1992. Vitrification of oocytes and embryos. En: Lauria AGF, editor. New trends in embryo transfer. Cambridge (UK). Portland Press; 255-264.
BERNAL, S.M.; HEINZMANN, J.; HERRMANN, D.; LUCAS-HAHN, A.; TIMMERMANN, B.; NIEMANN, H. 2012. Effects of different in vitro maturation systems on bovine embryo development. Repr., Fert. Developm. 23(1):210(Abstract).
BIELANSKI, A.; BERGERON, H.; LAU, P.C.; DEVENISH, J. 2003. Microbial contamination of embryos and semen during long term banking in liquid nitrogen. Cryobiology. 46(2):146-152.
BOLÍVAR, P.; MALDONADO, E.J. 2008. Análisis de costos de esquemas de transferencia de embriones bovinos utilizados en Colombia. Rev. Col. Cie. Pec. 21(3):351-364.
CABRERA, P.; FERNÁNDEZ, A.; BASTIDAS, P.; MOLINA, M.; BETHENCOURT, A.; DÍAZ, T. 2006. Vitrificación: Una Alternativa para la Criopreservación de Embriones. Rev. Fac. Cie. Vet. 47(1):9-23.
CAMPOS-CHILLON, L.F.; BARCELO, T.K.F.; SEIDEL, J.R.G.; CARNEVALE, E. 2009. Vitrification of earlystage bovine and equine embryos. Theriogenology. 71(2):349-354.
CELESTINOS, M.; GATICA, R. 2002. Vitrificación como técnica de crioconservación de embriones bovinos. Arch. Med. Vet. 34(2):157-165.
CHACÓN, J.L. 2001. Nacen primeros terneros in vitro en Colombia. Agr. Américas. 302:37-38.
DATTENA, M.; ACCARDO, C.; PILICHI, S.; ISACHENKO, V.; MARA, L.; CHESSA, B.; CAPPAI, P. 2004. Comparison of different vitrification protocols on viability after transfer of ovine blastocysts in vitro produced and in vivo derived. Theriogenology. 62(3- 4):481-493.
DE LA FUENTE, R.; KING, W.A. 1997. Use of a chemically defined system for the direct comparison of inner cell mass and trophectoderm distribution in murine, porcine and bovine embryos. Zygote. 5(4):309-320.
DOBRINSKY, J.R.; HESS, F.F.; DUBY, R.T.; JROBL, J.R. 1991. Cryopreservation of bovine embryos by vitrification. Theriogenology. 37:202(Abstract).
DONNAY, I.; AUQUIER, P.H.; KAIDI, S.; CAROLAN, C.; LONERGAN, P.; MERMILLOD, P.; MASSIP, A. 1998. Vitrification of in vitro produced bovine blastocysts: methodological studies and developmental capacity. An. Repr. Sci. 52(2):93-104.
ELDRIDGE-PANUSKA, W.D.; DI BRIENZA, V.C.; SEIDEL, J.R.; SQUIRES, E.; CARNEVALE, E.M. 2005. Establishment of pregnancies after serial dilution or direct transfer by vitrified equine embryos. Theriogenology. 63(5):1308-1319.
EL-GAYAR, M.; HOLTZ, W. 2001. Technical note: vitrification of goat embryos by the open pulled straw method. J. An. Sci. 79(9):2436-2438.
ELNAHAS, A.; ALCOLAK, E.; ABU MARAR, E.; ELNAHAS, T.; ELNAHAS, K.; PALAPELAS, V.; DIEDRICH, K.; AL-HASANI, S. 2010. Vitrification of human oocytes and different development stages of embryos: An overview. Middle East Fert. Soc. J. 15(1):2-9.
GÓMEZ, E.; MUÑOZ, M.; RODRÍGUEZ, A.; CAAMANO, J.N.; FACAL, N.; DIEZ, C. 2009. Vitrification of Bovine Blastocysts Produced In vitro Inflicts Selective Damage to the Inner Cell Mass. Repr. Dom. An. 44(2):194-199.
HANSEN, P.; BLOCK, J.; LOUREIRO, B.; BONILLA, L.; HENDRICKS, K. 2010. Effects of gamete source and culture conditions on the competence of in vitro - produced embryos for post-transfer survival in cattle. Repr., Fert. Developm. 22(1):59-66.
HIRAOKA, K.; HIRAOKA, K.; KINUTANI, M.; KINUTANI, K. 2004. Case report: successful pregnancy after vitrification of human blastocyst that had completely escaped from the zona pellucida on day 6. Hum. Repr. 19(4):988-990.
HUANG, J.; CHUNG, J.; LIN TAN, S.; CHIAN, R. 2007. High survival and hatching rates following vitrification of embryos at blastocyst stage: a bovine model study. Repr. BioMed. 14(4):464-470.
HAMAWAKI, A.; KUWAYAMA, M.; HAMANO, S. 1999. Minimum volume cooling method for bovine blastocyst vitrification. Theriogenology. 51(1):165 (Abstract).
INABA, Y.; AIKAWA, Y.; HIRAI, T.; HASHIYADA, Y.; YAMANOUCHI, T.; MISUMI, K.; OHTAKE, M.; SOMFAI, T.; KOBAYASHI, S.; SAITO, N.; MATOBA, S.; KONISHI, K.; IMAI, K. 2011. In straw cryoprotectant dilution for bovine embryos vitrified using cryotop. J. Repr. Developm. 57(4):437-443.
I WASAKI, S.; YOSHIBA, N.; USHIJIMA, H.; WATANABE, S.; NAKAHARA, T. 1990. Morphology and proportion of inner cell mass of bovine blastocysts fertilized in vitro and in vivo. J. Repr. Fert. 90(1):279-284.
KASAI, M. 1996. Simple and efficient methods for vitrification of mammalian embryos. An. Repr. Sci. 42(1-4):67-75.
KUWAYAMA, M.; HAMANO, S.; NAGAI, T. 1992. Vitrification of bovine blastocysts obtained by in vitro culture of oocytes matured in vitro. J. Repr. Fert. 96(1):187-193.
KUWAYAMA, M.; VAJTA, G.; LEDA, S.; KATO, O. 2005. Comparison of open and closed methods for vitrification of human embryos and the elimination of potential contamination Repr. Biomed. 11(5):608- 614.
LANE, M.; SCHOOLCRAFT, W.B.; GARDNER, D.K. 1999. Vitrification of mouse and human blastocysts using a novel cryoloop container-less technique. Fert. Ster. 72(6):1073-1078.
LEIBO, S.P.; MARTINO, A.; KOBAYASHI, J.; POLLAR, J.W. 1996. Stage-dependent sensitivity of oocytes and embryos to low temperatures. An. Repr. Sci. 42(1):45-53.
LEWIS, I.M.; LANE, M.W.; VAJTA, G. 1999. Pregnancy rates following transfer of in vitro produced bovine embryos vitrified by the open pulled straw (OPS) method. Theriogenology. 51(1):168 (Abstract).
LIEBERMANN, J.; NAWROTH, F.; ISACHENKO, V.; ISACHENKO, E.; RAHIMI, G.; TUCKER, M.J. 2002. Potential Importance of vitrification in reproductive medicine. Biol. Repr. 67(6):1671-1680.
LUCENA, E.; BERNAL, D.P.; LUCENA, C.; ROJAS, A.; MORAN, A.; LUCENA, A. 2006. A successful ongoing pregnancies after vitrification of oocytes. Fert. Ster. 85(1):108-111.
MANJUNATHA, B.; GUPTA, P.; RAVINDRA, J.; DEVARAJA, M.; NANDI, S. 2009. Effect of vitrification medium composition and exposure time on post- thaw development of buffalo embryos produced in vitro. The Vet. J. 179(2):287-291.
MASSIP, A.; MERMILLOD, P.; DINNYES, A. 1995. Morphology and biochemistry if in-vitro- produced bovine embryos: implications for their cryopreservation. Hum. Repr. 10(11):3004-3011.
MARTÍNEZ, A.G.; VALCÁRCEL, A.; DE LAS HERAS, M.A.; DE MATOS, D.G.; FURNUS, C.; BROGLIATTI, G. 2002. Vitrification of in vitro produced bovine embryos: in vitro and in vivo evaluations. An. Repr. Sci. 73(1):11-21.
MARTÍNEZ, A.G.; VALCARCEL, A.; FURNUS, C.C.; DE MATOS, D.G.; IORIO, G.; DE LAS HERAS, M.A. 2006. Cryopreservation of in vitro-produced ovine embryos. Small Rum. Res. 63(3):288-296.
MATSUMOTO, H.; JIANG, J.Y.; TANAKA, T.; SASADA, H.; SATO, E. 2001. Vitrification of large quantities of immature bovine oocytes using nylon mesh. Cryobiology. 42(2):139-144
MIYAKE, T.; KASAI, M.; ZHU, S.E.; SAKURAI, T.; MACHIDA, T. 1993. Vitrification of mouse oocytes and embryos at various stages of development in an ethylene glycol-based solution by a simple method. Theriogenology. 40(1):121-134.
NEDAMBALE, T.; DINNYE'S, A.; GROEN, W.; DOBRINSKY, J.; TIAN, X.; YANG, X. 2004. Comparison on in vitro fertilized bovine embryos cultured in KSOM or SOF and cryopreserved by slow freezing or vitrification. Theriogenology. 62(3-4):437- 449.
NGUYEN, B.X.; SOTOMARU, Y.; TANI, T.; KATO, Y.; TSUNODA, Y. 2000. Efficient cryopreservation of bovine blastocysts derived from nuclear transfer with somatic cells using partial dehydration and vitrification. Theriogenology. 53(7):1439-1448.
OHBOSHI, S.; FUJIHARA, N.; YOSHIDA, T.; TOMOGANE, H. 1997. Usefulness of polyethylene glycol for cryopreservation by vitrification of in vitro derived bovine blastocysts. An. Repr. Sci. 48(1):27- 36.
PALASZ, A.T.; MAPLETOFT, R.J. 1996. Cryopreservation of mammalian embryos and oocytes: recent advances. Biotech. Adv. 14(2):127-149.
PALASZ, A.T.; GUSTAFSSON, H.; RODRIGUEZ- MARTINEZ, H.; GUSTA, L.; LARSSON, B.; MAPLETOFT, R.J. 1997. Vitrification of bovine ivf blastocysts in an ethylene glycousucrose solution and heat-stable plant-extracted proteins. Theriogenology. 47(4):865-879.
PALMA, G. 2008. Biotecnología de la Reproducción. 2ª ed. Ed Reprobiotec, Buenos Aires, Argentina. 669p.
PARK, S.P.; KIM, E.Y.; KIM, D.I.; PARK, N.H.; WON, Y.S.; YOON, S.H.; CHUNG, K.S.; LIM, J.H. 1999. Simple, efficient and successful vitrification of bovine blastocysts using electron microscope grids. Hum. Repr. 14(11):2838-2843.
PARK, S.Y.; KIM, E.Y.; CUI, X.S.; TAE, J.C.; LEE, W.D.; KIM, N.H.; PARK, S.P.; LIM, J.H. 2006. Increase in DNA fragmentation and apoptosis-related gene expression in frozen-thawed bovine blaslocysts. Zygote. 14(2):125-131.
PILTTI, K.; LINDEBERG, H.; AALTO, J.; KORHONEN, H. 2004. Live cubs born after transfer of OPS vitrified- warmed embryos in the farmed European polecat (Mustela putorius). Theriogenology; 61(5):811-820.
PONTES, J.H.; SILVA, K.; BASSO, A.; RIGO, A.; FERREIRA, C.; SANTOS, G.; SANCHES, B.; PORCIONATO, J.; VIEIRA, P.; FAIFER, F.; STERZA, F.; SCHENK J.; SENEDA, M. 2010. Large-scale in vitro embryo production and pregnancy rates from Bos taurus, Bos indicus, and indicus-taurus dairy cows using sexed sperm Theriogenology. 74(8):1349-1355.
RALL, W.F.; FAHY, G.M. 1985. Ice free cryopreservation of mouse embryos at -196°C by vitrification. Nature. 313:573575.
RALL, W.F. 1987. Factors affecting the survival of mouse embryos cryopreserved by vitrification. Cryobiology. 24(5):387-402.
RÍOS, G.L.; MUCCI, N.C.; KAISER, G.G.; ALBERIO, R.H. 2010. Effect of container, vitrification volume and warming solution on cryosurvival of in vitro- produced bovine embryos. An. Repr. Sci. 118(1):19- 24.
RIZOS, D.; GUTIERREZ-ADAN, A.; PEREZ-GARNELO, S.; DE LA FUENTE, J.; BOLAND, M.P.; LONERGAN, P. 2003. Bovine embryo culture in the presence or absence of serum: implications for blastocyst development, cryotolerance, and messenger RNA expression. Biol. Repr. 68(1):236-243.
RIZOS, D.; CLEMENTE, M.; BERMEJO-ALVAREZ, P.; DE LA FUENTE, J.; LONERGAN, P.; GUTIERREZ- ADAN, A. 2008. Consequences of In vitro culture conditions on embryo development and quality. Repr. Dom. An. 43(4):44-50.
RODRÍGUEZ, P.; JIMÉNEZ, C. 2011. Criopreservación de embriones bovinos producidos in vitro. Rev. Fac. Med. Vet. Zoot. 58(2):107-119.
SAHA, S.; OTOI, T.; TAKAGI, M.; BOEDIONO, A.; SUMANTRI, C.; SUZUKI, T. 1996. Normal calves obtained after direct transfer of vitrified bovine embryos using ethylene glycol, trehalose and polyvinylpyrrolidone. Cryobiology. 33(3):291-299.
SARAGUSTY, J.; ARAV, A. 2011. Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification. Repr. 141(1):1-19.
SERRANO, C.; SIERRA, R.; SÁNCHEZ, J.; RESTREPO, L. OLIVERA, M. 2002. Evaluación de dos métodos de criopreservación sobre la calidad de embriones producidos in vitro. Rev. Col. Cie. Pec. 15(3):286- 292.
SOMMERFELD, V.; NIEMANN, H. 1999. Cryopreservation of bovine in-vitro produced embryos using ethylene glycol in controlled freezing or vitrification. Cryobiology. 38(2):95-105.
STINSHOFF, H.; WILKENING, S.; HANSTEDT, A.; BRÜNING, K.; WRENZYCKI, C. 2011. Cryopreservation affects the quality of in vitro produced bovine embryos at the molecular level. Theriogenology. 76(8):1433-1441.
STROUD, B. 2011. Statistics and data retrieval Committee report. The year 2010 worldwide statistics of embryo transfer in domestic farm animals. Disponible desde Internet en: http://www.iets.org/pdf/December2011.pdf (con acceso 01/07/2012).
TOMINAGA, K.; HAMADA, Y. 2001. Gel-loading tips as a container for vitrification of in vitro-produced bovine embryos. J. Repr. Developm. 47(5):267-273.
VAJTA, G.; HOLM, P.; GREVE, T.; CALLESEN, H. 1996. Factors affecting survival rates of in vitro produced bovine embryos after vitrification and direct in-straw rehydration. An. Repr. Sci. 45(3):191-200.
VAJTA, G.; HOLM, P.; GREVE, T.; CALLESEN, H. 1997. Comparison of two manipulation methods to produce in vitro fertilized, biopsied and vitrified bovine embryos. Theriogenology. 47(2):501-509.
VAJTA, G.; HOLM, P.; KUWAYAMA, M.; BOOTH, P.J.; JACOBSEN, H.; GREVE, T.; CALLESEN, H. 1998. Open pulled straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol. Repr. Developm. 51(1):53-58.
VAJTA, G.; RINDOM, N.; PEURA, T.; HELM, P.; GREVE, T.; CALLESEN, H. 1999. The effect of media, serum and temperature on in vitro survival of bovine blastocyst after open pulled straw (OPS) vitrification. Theriogenology. 52(5):939-949.
VAJTA G. 2000. Vitrification of the oocytes and embryos of domestic animals. An. Repr. Sci. 60-61:357-364.
VAJTA, G.; KUWAYAMA, M. 2006. Improving cryopreservation systems. Theriogenology. 65(1):236-244.
VAJTA, G. 2012. Vitrification in human and domestic animal embryology: work in progress. Repr., Fert. Developm. Disponible desde Internet en: http://dx.doi.org/10.1071/RD12118 (con acceso el 01/07/2012).
VARAGO, F.; SALIBA, W.; ALVIM, M.; VASCONCELOS, A.; OLIVEIRA, C.; STAHLBERG, R.; LAGARES, M. 2006. Vitrification of in vitro produced Zebu embryos. An. Repr. 3:353-358.
VANDERZWALMEN, P.; BERTIN, G.; DEBAUCHE, C.; STANDAERT, V.; VAN ROOSENDAAL, E.; VANDERVORST, M.; BOLLEN, N.; ZECH, H.; MUKAIDA, T.; TAKAHASHI, K.; SCHOYSMAN. R.; 2002. Births after vitrification at morula and blastocyst stages: effect of artificial reduction of the blastocoelic cavity before vitrification. Hum. Repr. 17:744-751.
VAN WAGTENDONK DE-LEEUW, A.M.; DEN-DAAS, J.H.G.; RAIL, W.F. 1997. Field trial to compare pregnancy rates of bovine embryo cryopreservation methods: vitrification and one-step dilution versus slow freezing and three-step dilution. Theriogenology. 48(7):1071-1084.
VIEIRA, A.D.; FORELL, F.; FELTRIN, C.; RODRIGUES, J.L. 2007. In-straw cryoprotectant dilution of IVP bovine blastocysts vitrified in hand-pulled glass micropipettes. An. Repr. Sci. 99(3-4):377-383.
VISINTIN, J.A.; MARTINS, J.P.F.; BEVILACQUA, E.M.; MELLO, M.R.; NICACIO, A.C.; ASSUMPCAO, M.A. 2002. Cryopreservation of Bos taurus vs Bos indicus embryos: are they really different? Theriogenology. 57(1):345-359.
WALKER, D.J.; CAMPOS-CHILLON, L.F.; SEIDEL, G.E. 2006. Vitrification of in vitro-produced bovine embryos by addition of ethylene glycol in one-step. Repr. Dom. An. 41(5):467-471.
WURTH, Y.A.; REINDERS, J.M.C.; RALL, W.F.; KRUIP, T.A.M. 1994. Developmental potential of in vitro produced bovine embryos following cryopreservation and single-embryo transfer. Theriogenology. 42(8):1275-1284.
XU, J.; GUO, Z.; SU, L.; NEDAMBALE, T.L.; ZHANG, J.; SCHENK, J.; MORENO, J.F.; DINNYÉS, A.; JI, W.; TIAN, X.C.; YANG, X.; DU, F. 2006. Developmental potential of vitrified Holstein cattle embryos fertilized In vitro with sex-sorted sperm. J. Dairy Sci. 89(7):2510-2518.
YAVIN, S.; ARAV, A. 2007. Measurement of essential physical properties of vitrification solutions. Theriogenology. 67(1):81-89.
YU, X.L.; DENG, W.; LIU, F.J.; LI, Y.H.; LI, X.X.; ZHANG, Y.L.; ZAN, L.S. 2010. Closed pulled straw vitrification of in vitro-produced and in vivo-produced bovine embryos. Theriogenology. 73(4):474-479.
ZERON, Y.; PEARL, M.; BOROCHOV, A.; ARAV, A. 1999. Kinetic and temporal factors influence chilling injury to germinal vesicle and mature bovine oocytes. Cryobiology. 38(1):35-42.
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