Response of Bradyrhizobium japonicum to alginate in presence of pelleted fungicides on soybean seeds
Respuesta de Bradyrhizobium japonicum a la adición de alginato en presencia de fungicidas peletizados en semillas de soya
Main Article Content
Abstract
The aim of this research was to determine the response of an inoculant based on strains of Bradyrhizobium japonicum with the addition of alginate in presence of Carbendazim® and Fludioxonil® pelleted fungicides in soybean seeds. The experiment consisted of eight treatments completely randomized with a 3x2 factorial arrangement with three replications by triplicate. Estimation of bacterial survival, as response variable, was performed at three times of drying: 0, 24 and 48 hours. Data were expressed as Log10 (CFU/ mL). Results showed that the two fungicides significantly reduce (p<0.05) the viability of B . japonicum. Fludioxonil® presented a stronger inhibitory effect than Carbendazim® with more than 25% of reduction at 48 hours. Moreover, it was shown that alginate promotes the adherence over soybean seeds and bacteria protection, diminishing the fungicide inhibitory action (p<0.05) after 24 hours. These results suggest that application of polymers in bacteria inoculants are a promising alternative to reduce negative effects of fungicides over cellular viability.
Keywords:
Downloads
Publication Facts
Reviewer profiles N/A
Author statements
- Academic society
- Universidad de Ciencias Aplicadas UDCA
- Publisher
- Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
Article Details
References (SEE)
AHEMAD, M.; KHAN, M.S. 2012a. Alleviation of fungicide-induced phytotoxicity in greengram [Vigna radiata (L.) Wilczek] using fungicide-tolerant and plant growth promoting Pseudomonas strain. Saudi J. Biol. Sci. (Saudi Arabia). 19(4):451-459.
AHEMAD, M.; KHAN, M.S. 2012b. Effect of pesticides on plant growth promoting traits of greengram- symbiont, Bradyrhizobium sp. strain MRM6. Bull. Environ. Contam. Toxicol. (United States). 86(4):384-388.
BASHAN, Y.; BASHAN, L.E.; PRABHU, S.R.; HERNANDEZ, J.P. 2013. Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998-2013). Plant and Soil. (Netherlands). 378(1-2):1-33.
CASSÁN, F.; PERRIGA, D.; SGROYA, V.; MASCIARELLIA, O.; PENNAB, C.; LUNA, V. 2009. Azospirillum brasilense Az39 and Bradyrhizobium japonicum E109, inoculated singly or in combination, promote seed germination and early seedling growth in corn (Zea mays L.) and soybean (Glycine max L.). Eur. J. Soil Biol. (Italy). 45:28-35.
CORTÉS-PATIÑO, S.; BONILLA, R. 2015. Polymers selection for a liquid inoculant of Azospirillum brasilense based on the Arrhenius thermodynamic model. Afr. J. Biotechn. (Kenya). 14(33):2547-2553.
COVARRUBIAS, S.A.; DE-BASHAN, L.E.; MORENO, M.; BASHAN, Y. 2012. Alginate beads provide a beneficial physical barrier against native microorganisms in wastewater treated with immobilized bacteria and microalgae. Appl. Microbiol. Biotechnol. (Germany). 93:2669-2680.
DAYAMANI, K.J.; BRAHMAPRAKASH, G.P. 2014. Influence of form and concentration of the osmolytes in liquid inoculants formulations of plant growth promoting bacteria. Int. J. Scient. Res. Publ. (Malaysa). 4(7):1-6.
DESHWAL, V.K.; SINGH, S.B.; KUMAR, P.; CHUBEY A. 2013. Rhizobia unique plant growth promoting Rhizobacteria: A Review. Int. J. Life Scienc. (Nepal). 2(2):74-86.
DOYLE, M.; BEUCHAT, L.; MONTVILLE, T. 2001. Microbiología de los alimentos fundamentos y fronteras. 1 ed. Ed. Acribia (España). 816p.
GIONGO, A.; BENEDUZI, A.; GANO, K.; VARGAS, L.K.; UTZ, L.; PASSAGLIA, L.M.P. 2013. Characterization of plant growth-promoting bacteria inhabiting Vriesea gigantea Gaud. and Tillandsia aeranthos (Loiseleur) L.B. Smith (Bromeliaceae). Biota Neotrop. (Brazil). 13(3):80-85.
GOMBOTZ, W.R.; WEE, S.F. 2012. Protein release from alginate matrices. Advanc. Drug Delivery Rev. (Netherlands). 64:194-205.
IMFELDA, G.; VUILLEUMIERB, S. 2012. Measuring the effects of pesticides on bacterial communities in soil: A critical review. Eur. J. Soil Biol. 49:22-30.
JIANYA, S.U.; YE-CHENG, W.; SHU-KUN, Z.; XIU-BEI, R. 2014. Antifungal agents against Aspergillus niger for rearing rice leaffolder larvae (Lepidoptera: Pyralidae) on artificial diet. J. Econ. Entomol. (United States). 107(3):1092-1100.
JOHN, R.P.; TYAGI, R.D.; BRAR, S.K.; SURAMPALLI, R.Y.; PREVOST, D. 2011. Bio-encapsulation of microbial cells for targeted agricultural delivery. Crit. Rev. Biotechnol. (United Kingdom). 31:211-226.
KEUM, Y.S.; LEE, H.R.; KIM, J.H. 2010. Effects of pesticides on the bacterial production of pyrrolnitrin. J. Agric. Food Chem. (Nigeria). 58(9):5531-5537.
LEE, K.Y.; MOONEY, D.J. 2012. Alginate: Properties and biomedical applications. Progress in Polymer Science. (United Kingdom). 37(1):106-126.
LO, C.C. 2010. Effect of pesticides on soil microbial community. J. Environ. Sci. Health B. (United Kingdom). 45:348-359.
MIÑAMBRES, G.G.; CONLES, M.Y.; LUCINI, E.I.; VERDENELLI, R.A.; MERILES, J.M.; ZYGADLO, J.A. 2010. Application of thymol and iprodione to control garlic white rot (Sclerotium cepivorum) and its effect on soil microbial communities. World J. Microbiol. Biotechnol. (Netherlands). 26(1):161-170.
MISHRA, G.; KUMAR, N.; GIRI, K.; PANDEY, S. 2013. In vitro interaction between fungicides and beneficial plant growth promoting rhizobacteria. Afr. J. Agr. Res. (Nigeria). 8(45):5630-5633.
NGUYEN, T.H.N.; BRECHENMACHER, L.; ALDRICH, J.T.; CLAUSS, T.R.; GRITSENKO, M.A.; HIXSON, K.K.; LIBAULT, M.; TANAKA, K.; YANG, F.; YAO, Q.; PASA-TOLIC, L.; XU, D.; NGUYEN, H.T.; STACEY, G. 2012. Quantitative phosphoproteomic analysis of soybean root hairs inoculated with Bradyrhizobium japonicum. Mol. Cell Proteomics. (United States). 11:1140-1155.
PEREIRA, C.E.; MOREIRA, F.M. de S.; OLIVEIRA, J.A.; CALDEIRA, C.M. 2010. Compatibility among fungicide treatments on soybean seeds through film coating and inoculation with Bradyrhizobium strains. Agronomy (United States). 32(4):585-589.
PII, Y.; MIMMO, T.; TOMASI, N.; TERZANO, R.; CESCO, S.; CRECCHIO, C. 2015. Microbial interactions in the rhizosphere: beneficial influences of plant growth- promoting rhizobacteria on nutrient acquisition process. A review. Biol. Fertil. Soils (Germany). 51(4):403-415.
RIVERA, D.; OBANDO, M.; BARBOSA, H.; ROJAS- TAPIAS, D.; BONILLA, R. 2014. Evaluation of polymers for the liquid rhizobial formulation and their influence in the Rhizobium-Cowpea interaction. Universitas Scientiarum. (Colombia). 19(3):265-275.
ROJAS-TAPIAS, D.; VERA, M.; RIVERA, D.; KLOEPPER, J.; BONILLA, R. 2013. Evaluation of three methods for preservation of Azotobacter chroococcum and Azotobacter vinelandii. Universitas Scientiarum. (Colombia). 18(2):129-139.
ROJAS-TAPIAS, D.; ORTEGA, O.; RIVERA, D.; BONILLA, R. 2015. Preservation of Azotobacter chroococcum vegetative cells in dry polymers. Universitas Scientiarum. (Colombia). 20(2):201-207.
ROJAS-TAPIAS, D.; MORENO-GÁLVAN, A.; PARDO- DÍAZ, S.; OBANDO, M.; RIVERA, D.; BONILLA, R. 2012. Effect of inoculation with plant growth- promoting bacteria (PGPB) on amelioration of saline stress in maize (Zea mays). Applied Soil Ecology. (Netherlands). 61:264-272.
ROMERO-PERDOMO, F.A.; MORENO-GALVÁN, A.; CAMELO-RUSINQUE, M.; BONILLA, R. 2015. Efecto de la carragenina sobre Azotobacter chroococcum en semillas de algodón peletizadas con un fungicida. Rev. Agron. Noroeste Argent. (Argentina). 35(1):29-32.
RUBENS, J.C.; SILVA, R.; HUNGRIA, M. 2009. Nitrogen fixation with the soybean crop in Brazil: Compatibility between seed treatment with fungicides and bradyrhizobial inoculants. Symbiosis. (Netherlands). 48(1-3):154-163.
VALENCIA, R.A.; LIGARRETO, G. 2010. Análisis de la interacción soya-cepa (Bradyrhizobium japonicum) x ambiente, en oxisoles de la orinoquia colombiana. Agron. Col. (Colombia). 28(3):361-371.
VELOUKAS, T.; KALOGEROPOULOU, K.; MARKO- GLOU, A.N.; KARAOGLANIDIS, G.S. 2014. Fitness and competitive ability of Botrytis cinerea field isolates with dual resistance to SDHI and QoI fungicides, associated with several sdhB and the cytb G143A mutations. Phytopathology. (United States). 104(4):347-356.
VINCENT, L.M. 1970. A manual for the practical study of root-nodule bacteria. IBP Handbook no. 15. Ed. Blackwell Scientific (United Kingdom). 164p.
ZHANG, Y.; LAMM, R.; PILLONEL, C.; LAM, S.; XU, J.R. 2002. Osmoregulation and fungicide resistance: the Neurospora crassa os-2 gene encodes a HOG1 mitogen-activated protein kinase homologue. Appl. Environ. Microbiol. (United States). 68:532-538.